説明
The MycoPins method is a protocol to monitor early colonization events in communities of wood-inhabiting fungi in fine woody debris. The sampling dataset presented here is based on fieldwork from a time series experiment on standard sterilized poplar pins (furniture wooden dowels 1 cm diameter, 3 cm length) that were placed in soil to decay, then collected and subjected to DNA metabarcoding analysis and automated molecular identification of species. The list of species for each sampling point during the duration of the experiment was produced by a taxonomic classifier PROTAX (Abarenkov, 2018). PROTAX analyses the DNA sequences and provides with a table of reliably identified (probability >0.9) species, which is about 10% of the overall sequences from our experiment. The list of published species is limited to only 10% because while identifying the species, PROTAX takes into the account the uncertainty and mislabelings in the UNITE database that was used as a reference.
データ レコード
この sampling event リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、15 レコードが含まれています。
拡張データ テーブルは1 件存在しています。拡張レコードは、コアのレコードについての追加情報を提供するものです。 各拡張データ テーブル内のレコード数を以下に示します。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は Kean University。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: 94d0f0a0-183f-49ae-aa8c-1bc4e930ec48が割り当てられています。 GBIF-US によって承認されたデータ パブリッシャーとして GBIF に登録されているKean University が、このリソースをパブリッシュしました。
キーワード
samplingEvent
連絡先
- メタデータ提供者 ●
- 論文著者 ●
- 最初のデータ採集者 ●
- 連絡先
- Associate Professor
http://orcid.org/0000-0001-7916-462X
- 最初のデータ採集者
- Assistant Professor
- 最初のデータ採集者
- Student
- 最初のデータ採集者
- Student
地理的範囲
Central New Jersey, Northeastern USA
| 座標（緯度経度） | 南 西 [40.695, -74.271], 北 東 [40.718, -74.234] |
|---|
生物分類学的範囲
説明がありません
| Kingdom | Fungi |
|---|
時間的範囲
| 開始日 / 終了日 | 2020-11-23 / 2021-05-02 |
|---|
プロジェクトデータ
MycoPins is a straightforward method for studying and monitoring dead wood colonization by fungi. The method combines standardized fieldwork sampling that includes experimental exposure of wooden pins to lignicolous fungi in the environment, followed by subsequent metabarcoding analysis of fungal DNA from the colonized pins.
| タイトル | MycoPins: a metabarcoding-based method to monitor fungal colonization of fine woody debris |
|---|---|
| Study Area Description | The study was concluded in a suburban area of central New Jersey, USA, from December 2020 to May 2021. |
プロジェクトに携わる要員:
- 論文著者
- 論文著者
収集方法
Sterilized poplar pins (wooden dowels) 1 cm in diameter and 3 cm long were placed in triplicates on November 23rd, 2020 in soil 2 cm from the surface, covered with debris and allowed to decay. Pins were extracted after 14, 28, 42, 77 and 160 days. Each pin was saved as an event, with parent events assigned to each date of extraction. Upon extraction, two pins for each triplicate were wrapped in brown paper and dried for 5 hours at 45°C in a conventional food dehydrator, and one pin was frozen at -80°C. Two sterile negative control pins were exposed to air in the field for 30 min and then one was dried and one frozen using the same methods used to store sample pins.The interior of each pin was drilled by a 2 mm fire-sterilized drill bit, DNA was isolated and PCR for the ITS2 gene region from the extracted DNA was carried out with the primers fITS7 forward (F), 5’-GTGARTCATCGAATCTTTG, and ITS4 reverse (R), 5’-TCCTCCGCTTATTGATATGC (Clemmensen, 2016). PCR amplicons were sequenced using an Illumina 2x 250 paired-end (PE) configuration.
| Study Extent | The sampling event was performed from December 2020 to May 2021 in a suburban area of central New Jersey, Northeast of USA. |
|---|---|
| Quality Control | During pre-processing of the sequencing data, raw pair-end sequences were merged using PEAR (Zhang et al, 2014), cutadapt (Martin 2011) was used for trimming and removing adapter/tag sequencing, and sequences were clustered using VSEARCH (Rognes et al, 2016) with 99% sequence similarity threshold. The resulting centroid sequences were processed with PROTAX (Abarenkov et al, 2018). Identification of species was performed using UNITE v 7.1 database. When calculating abundances of different taxa, the cluster sizes were taken into account. A sample-taxon table was produced as an output where the abundances were counted as the number of sequences whose taxon membership probability exceeded a 0.9 threshold (reliable identification). The resultant lists of species for each event (species in each pin) are shared in this dataset publication. |
Method step description:
- For the detailed step-by-step description, please see our recent paper: Shumskaya M., Lorusso N., Patel U., Leigh M., Somervuo P., Schigel D. (2023) "MycoPins: a metabarcoding-based method to monitor fungal colonization of fine woody debris". MycoKeys 96:77-95.
コレクションデータ
| コレクション名 | Kean University, Laboratory of Applied Genomics |
|---|
| 標本保存方法 | Deep frozen, Dried |
|---|
書誌情報の引用
- Clemmensen KE, Ihrmark K, Durling MB, Lindahl BD (2016) Sample Preparation for Fungal Community Analysis by High-Throughput Sequencing of Barcode Amplicons. Methods in molecular biology (Clifton, NJ) 1399: 61-88. doi:10.1007/978-1-4939-3369-3_4
- Martin M (2011) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet Journal 17: 10-12. doi:10.14806/ej.17.1.200
- Rognes T, Flouri T, Nichols B, Quince C, Mahe F (2016) VSEARCH: a versatile open source tool for metagenomics. Peerj 4. doi:10.7717/peerj.2584
- Zhang JJ, Kobert K, Flouri T, Stamatakis A (2014) PEAR: a fast and accurate Illumina Paired-End reAd mergeR. Bioinformatics 30: 614-620. doi:10.1093/bioinformatics/btt593
- Abarenkov K, Somervuo P, Nilsson RH, Kirk PM, Huotari T, Abrego N, Ovaskainen O (2018) PROTAX-fungi: a web-based tool for probabilistic taxonomic placement of fungal internal transcribed spacer sequences. New Phytologist 220: 517-525 doi:10.1111/nph.15301
- Shumskaya M, Lorusso N, Patel U, Leigh M, Somervuo P, Schigel D. (2023) MycoPins: a metabarcoding-based method to monitor fungal colonization of fine woody debris. doi: 10.3897/mycokeys.96.101033
追加のメタデータ
| 代替識別子 | 94d0f0a0-183f-49ae-aa8c-1bc4e930ec48 |
|---|---|
| https://doi.org/10.15468/r7rxf6 | |
| https://ipt.gbif.us/resource?r=mycopins |