Kenai National Wildlife Refuge Pollinator Surveys

最新バージョン United States Fish and Wildlife Service により出版 5月 5, 2023 United States Fish and Wildlife Service

DwC-A形式のリソース データまたは EML / RTF 形式のリソース メタデータの最新バージョンをダウンロード:

DwC ファイルとしてのデータ ダウンロード 3,986 レコード English で (739 KB) - 更新頻度: annually
EML ファイルとしてのメタデータ ダウンロード English で (18 KB)
RTF ファイルとしてのメタデータ ダウンロード English で (16 KB)


We collected pollinators as part of the Alaska Bee Atlas effort ( We collected pollinators on Kenai National Wildlife Refuge in 2022 using blue vane traps, pollinator cups, and aerial nets.

データ レコード

この オカレンス(観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、3,986 レコードが含まれています。

拡張データ テーブルは3 件存在しています。拡張レコードは、コアのレコードについての追加情報を提供するものです。 各拡張データ テーブル内のレコード数を以下に示します。

Occurrence (コア)

この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。





パブリッシャーとライセンス保持者権利者は United States Fish and Wildlife Service。 To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.


このリソースをはGBIF と登録されており GBIF UUID: 86875091-d166-4986-802a-343b341424c6が割り当てられています。   GBIF-US によって承認されたデータ パブリッシャーとして GBIF に登録されているUnited States Fish and Wildlife Service が、このリソースをパブリッシュしました。


Occurrence; bees; pollinators


Matthew Bowser
  • メタデータ提供者
  • 最初のデータ採集者
  • 連絡先
Fish and Wildlife Biologist
USFWS Kenai National Wildlife Refuge
PO Box 2139
99669 Soldotna
Anya Bronowski
  • 最初のデータ採集者
Biological Technician
U.S. Fish and Wildlife Service, Kenai National Wildlife Refuge
Dom Watts
  • 最初のデータ採集者
U.S. Fish and Wildlife Service, Kenai National Wildlife Refuge


We surveyed on the Kenai National Wildlife Refuge on the Kenai Peninsula, Alaska.

座標(緯度経度) 南 西 [59.62, -151.1], 北 東 [60.55, -150.2]


We surveyed for pollinators, mainly Hymenoptera, Diptera, Lepidoptera, and Coleoptera.

Class Insecta


開始日 / 終了日 2011-06-30 / 2022-08-26


In 2022 we collected pollinators as part of the Alaska Bee Atlas effort ( We collected pollinators on Kenai National Wildlife Refuge using blue vane traps, pollinator cups, and aerial nets.

タイトル Kenai National Wildlife Refuge Pollinator Surveys
Study Area Description We surveyed on the Kenai National Wildlife Refuge, Kenai Peninsula, Alaska.


We followed the guidance of the sampling plan of Fulkerson et al. (2021). Most of KNWR lies within lowest priority hexagons of Fulkerson et al. (2021), but the southernmost part of the Refuge lies within a medium priority hexagon. We prioritized sampling in this area, but access in this region is difficult. We surveyed only at Emerald Lake in this medium priority hexagon. We surveyed for insect pollinators at a variety of other sites on the Refuge, trying to sample in diverse habitats. We sampled dry, rocky slopes off of Skilak Lake Road following the advice of Justin Fulkerson. We accessed sites by road and floatplane.

Study Extent The study area was the Kenai National Wildlife Refuge and its immediate environs.

Method step description:

  1. Field Methods We sampled pollinators using bee bowl traps, blue vane traps, and aerial nets, generally following the field methods of Fulkerson et al. (2021) with the exception that we collected specimens into SK picglobal 99.9% pure propylene glycol.
  2. Specimen Processing Samples were stored in a -23°C freezer except when samples were being processed. Invertebrates were separated from debris by hand under a dissecting microscope. Care was taken to reduce possible cross-contamination of DNA among samples. We separated samples that were all or mostly bees from samples that were mostly flies and other invertebrates. We shipped 12 samples of bees to the Alaska Center for Conservation Science3, University of Alaska Anchorage, Anchorage, Alaska to be processed. We homogenized the remaining 19 samples plus one legacy bulk pollinator sample from a previous project (Bowser 2012) using a blender and cleaning between samples with DIY-DS cleaning solution as described by Buchner et al. (2021). Our sample homogenization protocol is included below. We homogenized samples using a Nutri Ninja QB3000SS blender (Euro-Pro Operating LLC 2015). DIY-DS recipe 20 g NaOH 20 g Alconox 15.1 g NaHCO3 267 ml 4.5% bleach deionized water to fill to 2 l Preparation 120 ml plastic cups should be washed with DIY-DS and rinsed before sampling. Finish by rinsing inside the 120 ml cup with deionized water. Hand dry 120 ml cup with paper towel. Homogenize samples Before running samples, rinse blender by running 100 ml of deionized water for 20 s. Pre-label a 10 ml plastic vial with the specimen GUID and add a barcode vial label. Also pre-label and add a barcode label to a 120 ml specimen cup. Clean forceps with DIY-DS. Take the label out of the original container with the cleaned forceps and place into the new 120 ml sample container. Add the contents of the sample vial to the blender. Rinse original sample vial with cold, clean propylene glycol and pour rinsate in the blender with the rest of the sample. Fill blender to 100 ml with cold, clean propylene glycol. Blend for 90 s. Using a new disposable pipette, fill the pre-labelled 10 ml plastic vial with about 9.5 ml of homogenate. Pour the rest of the sample into the pre-labeled 120 ml specimen cup. Rinse blender by running 100 ml tap water for 10 s. Wash blender by running 100 ml of DIY-DS for 10 s. Rinse this out in the lab sink with tap water. Rinse blender by running 100 ml deionized water for 10 s. We shipped 9 ml of homogenate from each of the 20 homogenized samples to Molecular Research Laboratory, Shallowater, Texas for metabarcoding.
  3. Molecular Methods We chose to use the mlCOIintF/jgHCO2198 (GGWACWGGWT GAACWGTWTA YCCYCC / TAIACYTCIG GRTGICCRAA RAAYCA) primer set of Leray et al. (2013) for PCR, targeting a 313 bp region of the COI DNA barcoding region. The mlCOIintF/jgHCO2198 primer pair was used with barcodes on the forward primer in 30–35 PCR cycles using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94°C for 3 minutes, followed by 30–35 cycles of 94°C for 30s, 53°C for 40 seconds and 72°C for 1 minute, after which a final elongation step at 72°C for 5minutes was performed. After amplification, PCR products were checked in 2% agarose gel to determine the success of amplification and the relative intensity of bands. Multiple samples were pooled together in equal proportions based on their molecular weight and DNA concentrations. Pooled samples were purified using calibrated Ampure XP beads. The pooled and purified PCR product was used to prepare an illumina DNA library. Sequencing was performed at MR DNA on a MiSeq following the manufacturer’s guidelines.
  4. Bioinformatics The bioinformatics pipeline was run on the Yeti supercomputer (USGS Advanced Research Computing 2021). We used the MetaWorks pipeline, version 1.11.3 (Porter and Hajibabaei 2022) with the RDP classifier (Wang et al. 2007) and the Eukaryote CO1 reference set for the RDP Classifier, version 4.0.1 (Porter and Hajibabaei 2018). We processed data in R, version 4.2.2 and 4.2.3 (R Core Team 2022, 2023) using the R packages ape, version 5.7-1 (Paradis and Schliep 2019); Biostrings, version 2.66.0 (Pagès et al. 2022); bold, version 1.2.0 (Chamberlain 2021a); ips, version 0.0.11 (Heibl 2008); msa, version 1.30.1 (Bodenhofer et al. 2015); openssl, version 2.0.6 (Ooms 2023a); reshape2, version 1.4.4 (Wickham 2007); ritis, version 1.0.0 (Chamberlain 2021b); and uuid, version 1.1-0 (Urbanek and Ts’o 2022). We compared our sequences to sequences from a local reference library (Bowser 2022a) using the vsearch --usearch_global command of vsearch, version 2.21.1 (Rognes et al. 2016).


目的 Pollinating insects provide important ecosystem services in Alaska (Fulkerson et al. 2021) and the pollinators themselves are wildlife that the Kenai National Wildlife Refuge (KNWR or the Refuge) was established in part to conserve (Kenai National Wildlife Refuge and US Fish & Wildlife Service, Alaska Regional Office, Division of Conservation Planning & Policy 2010). Because pollinators appear to be generally declining (Potts et al. 2010, Cameron et al. 2011, Koh et al. 2016), we wanted to begin documenting pollinator diversity on the Refuge. The Alaska Bee Atlas (Fulkerson et al. 2021, is a sampling program designed to provide information on the biodiveristy of pollinators in Alaska. In 2022, KNWR biologists participated in the Alaska Bee Atlas effort.
代替識別子 86875091-d166-4986-802a-343b341424c6